Bovine albumin
Albumen Powder
CAS: 9048-46-8
Molecular Formula: N/A
Bovine albumin - Names and Identifiers
Bovine albumin - Physico-chemical Properties
| Molecular Formula | N/A |
| Water Solubility | Soluble in water. |
| Solubility | Soluble in water, coagulate and precipitate protein when heated (above 60°C). |
| Appearance | White or white-like freeze-dried powder |
| Color | clear clear, colorless |
| Merck | 13,8542 |
| PH | 6.5-7.5 (1% in 0.15 M sodium chloride) |
| Storage Condition | 2-8°C |
| Stability | Stable. Incompatible with strong oxidizing agents and strong acids. Refrigerate. |
| MDL | MFCD00130384 |
| Physical and Chemical Properties | White or white-like freeze-dried powder, soluble in water, and solidified and precipitated protein when heated (above 60 ℃). |
| Use | Used as medical and health food, condiment |
Bovine albumin - Risk and Safety
| Hazard Symbols | Xn - Harmful |
| Risk Codes | R22 - Harmful if swallowed R52/53 - Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment. R21/22 - Harmful in contact with skin and if swallowed. |
| Safety Description | S24/25 - Avoid contact with skin and eyes. S61 - Avoid release to the environment. Refer to special instructions / safety data sheets. S36/37 - Wear suitable protective clothing and gloves. |
| WGK Germany | 3 |
| RTECS | MT6446000 |
| FLUKA BRAND F CODES | 10-21 |
| TSCA | Yes |
| HS Code | 35029000 |
| Toxicity | LD50 intravenous in monkey: > 12500mg/kg |
Bovine albumin - Introduction
Open Data Unverified Data
bovine serum albumin (BSA), a kind of globulin in bovine serum, contains 607 amino acid residues, has a molecular weight of 66.446kDa and an isoelectric point of 4.7. Bovine serum albumin is widely used in biochemical experiments.
Last Update：2022-01-01 08:50:08
Bovine albumin - Nature
Open Data Unverified Data
Storage Conditions 2-8°C
Solubility in PBS: >40 mg/mL
form lyophilized powder
Merck 13,8542
Stable. Incompatible with strong oxidizing agents and strong acids.
EPA chemical substance information bubins, blood serum(9048-46-8)
Last Update：2022-01-01 08:50:08
Bovine albumin - Chemical properties and methods of production
Open Data Unverified Data
chemical properties
white or off-white freeze-dried powder, soluble in water, precipitated by coagulation of proteins when heated (above 60 °c).
production method
bovine blood was used as raw material to separate the hemorrhagic clear, and ammonium sulfate was used for Fractional precipitation, followed by treatment with caprylic acid for purification.
Last Update：2022-01-01 08:50:09
Bovine albumin - Safety Information
Open Data Unverified Data
marking of dangerous goods Xn
Hazard category code 22
Safety instructions 24/25
WGK Germany 3
F 10-21
Last Update：2022-01-01 08:50:09
Bovine albumin - Main ingredients
Open Data Unverified Data
Protein
is the main component of bovine serum. In addition to proteins that can carry metal ions, fatty acids and hormones themselves, there are mainly albumins, globulins. Fibronectin cells promote cell attachment; Alpha 2 macroglobulin inhibits the action of trypsin; Fetal bovine serum containing fetuin promotes cell attachment; Transferrin can bind iron ions, reduce its toxicity and be utilized by cells.
polypeptide
platelet growth factor can promote cell division, is one of the main members of the polypeptide family, is the main cell proliferation factor; Fibroblast growth factor, epidermal growth factor, the content of nerve cell growth factor in serum is very small, but it also has a certain effect on cell growth.
hormone
The effects of hormones on cells are multifaceted.
- insulin: promotes cellular uptake of glucose and amino acids, and is involved in promoting cell division.
- insulin-like growth factor: it can bind to the insulin receptor expressed by cells, and thus has the same effect as insulin.
- growth hormone: cell proliferation promoting effect.
- other ingredients: amino acids, glucose, keto acids and other synthetic media for a variety of nutrients of little significance. Trace elements in a protein-bound state are of interest for cell culture.
Last Update：2022-01-01 08:50:10
Bovine albumin - Structure and Function
Open Data Unverified Data
component structure
The simple protein in bovine serum, which is the main component of blood (38g/100ml), has a molecular weight of 68KD. Isoelectric point 4.8. Nitrogen content of 16%, sugar content of 0.08%. Containing only sugar and has sugar Amine, fat content of only 0.2%. Albumin consists of 581 amino acid residues, of which 35 cysteines make up 17 disulfide bonds, with a free thiol at position 34 of the peptide chain. Albumin can bind to a variety of cations, anions, and other small molecules. Albumin in the blood mainly plays a role in maintaining osmotic pressure, PH buffering, carrier and nutrition. In the serum-free culture of animal cells, the addition of albumin can play a role of physiological and mechanical protection and carrier. Bovine serum albumin (BSA), also known as the fifth component, is a kind of globulin in bovine serum, contains 583 amino acid residues, the molecular weight is 66.430 kDa, the isoelectric point is 4.7. Bovine serum albumin is widely used in biochemical experiments, such as western blot as Blocking agent.
effect
BSA is generally used as a stabilizer in preservation solutions and reaction solutions for restriction or modification of enzymes because some enzymes are unstable or have low activity at low concentrations. After adding BSA, it may play a role of "protection" or "carrier", and many enzymes can greatly improve their activity after adding BSA. Enzymes that do not require the addition of BSA are generally not affected by the addition of BSA. For most substrate DNAs, BSA allows for more complete digestion and enables repeated cleavage. At 37 C, the enzyme digestion reaction is more than 1H, BSA can make the enzyme more stable, because in the reaction buffer without BSA, many restriction endonucleases can survive only 10 "20min or less at 37 °c. BSA, in turn, can bind metal ions and other chemicals in the buffer or substrate DNA that inhibit restriction enzyme activity.
buffer
- BSA is a stabilizer of enzymes, preventing the decomposition of enzymes and non-specific adsorption.
- BSA can reduce the denaturation of some enzymes, and can reduce the denaturation caused by some adverse environmental factors such as heating, surface tension and chemical factors. As for the mechanism of action, I am not very clear, it may be because there are 17 disulfide bonds in its structure, and a sulfhydryl group, the chemical reaction of the thiol group is very active, and the disulfide bond has the effect of antioxidant reduction, so it can be associated with a variety of cations, anion and small molecule binding.
- BSA can prevent the enzyme from adsorbing to the tube wall and losing.
Last Update：2022-01-01 08:50:11
Bovine albumin - Storage method
Open Data Unverified Data
The solution may be dissolved by adding 100 of water per 10g, or dissolved by PBS, depending on the application. Then they were divided into small branches. If no preservative is used, it can be stored in a constant temperature cabinet of minus 20 or 30 degrees Celsius, and if a preservative (such as 0.1% sodium azide) is used, it can be stored in an environment of 4 degrees Celsius above zero. It can be stored for several months without deterioration. Preservatives may affect the effect of bovine serum albumin, should be used with caution.
Last Update：2022-01-01 08:50:11
Bovine albumin - Reference Information
| EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
| Introduction | bovine serum albumin (BSA) is one of the most commonly used proteins in biochemical laboratory, too trivial to ignore its importance. Bovine serum albumin (BSA), also known as the fifth component, is a kind of globulin in bovine serum, contains 583 amino acid residues, the molecular weight is 66.430 kDa, the isoelectric point is 4.7. Bovine serum albumin is widely used in biochemical experiments, such as western blot as Blocking agent. |
| Application | bovine serum albumin (BSA), also known as the fifth component, is a kind of globulin in bovine serum, contains 607 amino acid residues, has a wide range of applications in biochemical experiments. Bovine serum albumin is generally used as a stabilizer in preservation solutions and reaction solutions for restriction or modification of enzymes to stabilize the enzyme activity and to prevent decomposition of the enzyme and non-specific adsorption. |
| function | BSA is generally used as a stabilizer in the preservation solution and reaction solution of restriction enzymes or modified enzymes, because some enzymes are unstable or have low activity at low concentrations. After adding BSA, it may play a role of "protection" or "carrier", and many enzymes can greatly improve their activity after adding BSA. Enzymes that do not require the addition of BSA are generally not affected by the addition of BSA. For most substrate DNAs, BSA allows for more complete digestion and enables repeated cleavage. At 37 C, the enzyme digestion reaction is more than 1H, BSA can make the enzyme more stable, because in the reaction buffer without BSA, many restriction enzymes can survive for 10-20min or less at 37 °c. BSA, in turn, can bind metal ions and other chemicals in the buffer or substrate DNA that inhibit restriction enzyme activity. |
| Use | Standard Grade bovine serum albumin (BSA, Standard Grade), which can meet the needs of most routine experiments, for example, it is used as an immune blocking agent, tissue cell (microbial animal and insect cells, etc.) culture nutrient and culture component, protein/enzyme stabilizer and protein quantitative standard. Diagnostic Grade bovine serum albumin (BSA,Diagnostic Grade) can meet most of the routine experimental requirements, such as use as an immune blocking agent, protein/enzyme stabilizer, diluent, carrier and protein standard. In addition, it can also be used for immunoassays, cell culture and hybridization experiments with high sensitivity requirements. used in biochemical research, genetic engineering and medical research. used as a health food, seasoning biochemical research; Genetic engineering and pharmaceutical research; Protein standard preparation; And other protein stabilizers. |
| production method | bovine blood was used as raw material to separate the hemorrhagic serum, which was fractionated with ammonium sulfate and then purified by treatment with caprylic acid. |
Last Update：2024-04-09 20:49:11
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